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Double-click the downloaded file to install the software. · Many factors have been shown to influence bacterial transfer between surfaces, including surface type, bacterial species, moisture level, pressure, and. Update. Star has been tested to meet all of the technical requirements to be compatible with. Windows 1. 0, 8. 1, Windows 8, Windows 7, Windows Vista, Windows Server 2. Windows. XP, 3. 2 bit and 6. Simply double- click the downloaded file to install it. Update. Star Free and Update. Star Premium come with the same installer. Update. Star includes support for many languages such as English, German, French, Italian, Hungarian, Russian and many more. You can choose your language settings from within the program. Inoculum Size Influences Bacterial Cross Contamination between Surfaces. Food Risk Analysis Institute, Rutgers University, New Brunswick, New Jersey 0. Corresponding author. Mailing address: Food Risk Analysis Institute, Rutgers University, 6. Dudley Rd., New Brunswick, NJ 0. Phone: (7. 32) 9. Fax: (7. 32) 9. 32- 6. E- mail: ude. sregtur. Received 2. 00. 3 May 2. Accepted 2. 00. 3 Sep 1. Copyright © 2. 00. American Society for Microbiology. This article has been cited by other articles in PMC. Abstract. Many factors have been shown to influence bacterial transfer between surfaces, including surface type, bacterial species, moisture level, pressure, and friction, but the effect of inoculum size on bacterial transfer has not yet been established. Bacterial cross contamination rates during performance of common food service tasks were previously determined in our laboratory using nalidixic acid- resistant Enterobacter aerogenes. Eight different transfer rates were determined, each involving a minimum of 3. The influence of source inoculum level on the percentage of bacteria transferred (percent transfer rates) and log. CFU per recipient surface was determined using statistical analysis. The effect of inoculum size on transfer rate was highly statistically significant (P < 0. P = 0. 1. 64. 3). Where inoculum size on the source was greater, transfer rates were lower, and where inoculum size on the source was less, transfer rates were higher. The negative linear trend was more obvious for activities that had a larger range of inoculum sizes on the source surface. This phenomenon has serious implications for research seeking to determine bacterial cross contamination rates, since the different transfer efficiencies that were previously shown to be associated with certain activities may actually be the result of differing initial inoculum levels. The initial inoculum size on the source and the amount of bacteria transferred must both be considered to accurately determine bacterial transfer rates. Microbial concentration plays an essential role in many microbial systems. It plays an essential role in regulating bioluminescence (8), antibiotic biosynthesis (1), virulence determination (2. Bacillus megaterium spores germinate faster when present at higher concentrations (4). The inoculum size of Clostridium botulinum affects time to detection and the fraction of samples that show growth (3. A threshold inoculum size for Listeria monocytogenes to initiate growth at suboptimal conditions has been established (1. Whether due to interaction between cells, statistical effects, or sensitivity of microbiological methods, initial inoculum levels can drastically affect experimental results. Many factors that influence the transfer of bacteria from surface to surface have been identified. Type of bacteria (1. However, the effect of the initial inoculum level on transfer efficiency has not been established. Research conducted in our laboratory to determine the effectiveness of gloves as a barrier to cross contamination identified inoculum size as a possible factor influencing the percent transfer rate (1. When inoculum size on hands was small, the percent transfer rate through gloves to lettuce was high, and when inoculum size on hands was large, the percent transfer rate was correspondingly low. This is in contrast to other cross contamination rates determined in our laboratory where inoculum size had no obvious effect (5). Through further analysis of data from both studies, we sought to determine whether a connection exists between inoculum size and the percent of bacteria transferred for other cross contamination rates and, if so, the nature of that connection. MATERIALS AND METHODSPreparation of cultures. The methods used for preparation of cultures were as previously published (5, 1. These were based on methods originally proposed by Zhao et al. A nonpathogenic, food- grade microorganism, Enterobacter aerogenes B1. A (3. 1), was used for all experiments. This E. aerogenes strain is resistant to nalidixic acid, which allows it to be enumerated in the presence of other microorganisms in food and resident bacteria on the hands of study participants. Raw, skinless, boneless, chicken breast meat and iceberg lettuce were obtained from a local supermarket. Control experiments showed that nalidixic acid- resistant E. E. aerogenes cells were grown overnight (1. C with shaking (1. Difco, Detroit, Mich.) containing 5. Sigma Chemical Co., St. Louis, Mo.). Cells were harvested by centrifugation (Micro 7; Fisher Scientific, Pittsburgh, Pa.) at 5,0. PBS; 0. 1 M, p. H 7. Fisher Scientific Co.). Cell pellets were resuspended in PBS and adjusted by a spectrophotometer (model UV1. Shimadzu Scientific Instruments, Columbia, Md.) to an A6. CFU/ml. Appropriate 1. PBS were made to determine the cell density of the inoculum and enumerate samples collected from various surfaces. One- tenth milliliter of the two lowest dilutions was then plated in duplicate on Mac. Conkey agar (Difco) containing 5. Pour plating was done in duplicate by mixing 1 ml of a sample with 1. E. aerogenes. Agar plates were incubated at 3. C for 2. 4 h prior to enumeration. Study participants. More than 6. 0 Rutgers University students and staff participated in the study to produce at least 3. Both hands of each participant were sampled so that handedness would not be a factor. Biosafety and human subject assurance. While Enterobacter species have been linked to disease outbreaks, the victims of such disease have been in a weakened or immunocompromised state. Outbreaks have been linked to contaminated IV fluid (1. Immunocompromised individuals and anyone with obvious cuts or abrasions on the hands were not allowed to participate in these studies. Each participant was informed about the general nature of the experimental procedures and signed a consent form prior to taking part in the experiments. University biosafety committee and human subject approvals were obtained prior to the initiation of this study. The Food and Drug Administration has suggested the use of E. Salmonella and E. U. S. Department of Agriculture to ferment reducing sugars in egg whites prior to drying to prevent Maillard browning. Volunteers' hands were also sampled at the end of the experiment, and less than 1 log CFU of bacteria was left on the hands (2. After the sampling process, volunteers were instructed to wash their hands before leaving the laboratory, which further reduced the amount of bacteria present. As part of our experimental protocol, samples were taken in various sites around the laboratory. Sink faucets, doorknobs, soap dispensers, a countertop, a pen the volunteers had used after the experiment, and the buttons on the elevator in the hall were sampled. In all cases, the amount of E. Contamination of chicken and hands. We inoculated 1. 50- g portions of chicken with 1. E. aerogenes suspension gradually, one drop at a time, over the entire surface of the breast. Samples were then held for 1. Prior studies with this organism used a 3. The participant then cut the chicken into small cubes (approximately 1 by 1 by 1 cm) on a clean, sterile plastic cutting board (American Chef, Bentonville, Ark.), which transferred E. One of the participant's hands was sampled using the glove juice method (2. The fingers of a sterile surgical glove (Fisher Scientific Co.) were filled with PBS (2. The hand was rubbed for 1 min by an investigator, and the sample was collected for enumeration. The participant handled three sterile spigots to simulate turning on a water faucet using the hand not sampled by the glove juice method. To standardize the level of hand contamination, the participant transferred the diced chicken from the cutting board to a tray back and forth three times prior to handling each of the three spigots. To determine the number of E. Briefly, an alginate swab (Fisher Scientific Co.) was moistened in 0. Two swabs were used to sample each spigot, and the swabs were dissolved in 4 ml of sodium citrate (1%) for 5 min while being intermittently agitated on a vortex. The sample was then diluted in PBS, and E. The other two spigots were used to recontaminate the hands of each participant in a manner simulating normal use. The rate of cross contamination between metal spigot surfaces and hands (see below) was evaluated under two conditions: (i) when the participant's hands had some level of E. E. aerogenes negative). Lana Del Rey Must Think We're Idiots. 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Old school: skeezy dude on the subway pleasuring himself in public. New school: skeezy dude on the subway. Well, I can tell you from my own personal experience, ain’t nothing in this world smells, tastes and feels better that your own mom’s pussy! Metaphors? That would explain why there is no white Ford Mustang in the video, then. Perhaps I was taking things too literally. ![]() Silly me. I guess it would fit. The long- haired musician (who kind of looks like the fake hippie guy) being a wild horse, I mean. She thought she could soften him, but he, as a wild mustang, could not be tamed. It just wasn’t in his nature. And also he is white. So there’s that. I don’t know. That seems a little too on- the- nose to me. Or she’s talking about his dick. Who’s to say. (h/t to @teenlibido!). We See You And We Love You. Hey, you! You in that white BMW E6. M5 with the single greatest vanity license plate ever. We see you. And we love you (and your car). This M5 was spotted by reader TFritsch doesn’t even like cars 9. But oh, that 1%., who was at Mazda Raceway Laguna Seca this past Saturday. And it just warms my heart so. Besides the kickass license plate, we also spot a very subtle trunk lip- spoiler with Laguna Seca and Thunderhill Raceway stickers adorning the back window. And! Aftermarket exhaust tips, which almost certainly mean an aftermarket exhaust system. Because everybody wants to hear that 5. V1. 0 SING. Whoever you are, you are a class- fuckin’- act, my friend. Update #1: A twist! User Alexioflexio wrote in to say that the reason the M5's Jalopnik spelling used a “4" instead of an “A” was because he stole the vanity plate first: This could not make me any happier to see. A Jalopnik vanity plate on a wagon! Tell me that Mc. Laren is going to get similar treatment. Update #2: Reader Flynorcal, pink panther pantry thief, dehydrates easily has spoken! The white M5 does indeed belong to him. Here’s a photo of the front of it. Do I spot blacked- out side- and kidney grilles as well? ![]() Organizer of Neo- Nazi Rally Tweets That Death of Heather Heyer Was 'Payback Time' [Updated]Jason Kessler, the organizer of last weekend’s neo- Nazi rally in Charlottesville and a former contributor to the Daily Caller, tweeted overnight that the death of Heather Heyer was “payback time.” Response in the white nationalist community was mixed, with some distancing themselves from Kessler, others applauding his tweet, and still others floating various conspiracy theories. Kessler’s tweet referred to Heather Heyer, the woman killed by a neo- Nazi in Charlottesville, as a “fat disgusting Communist” and claimed that since Communists had “killed 9. His tweet included a link to the Daily Stormer, a white supremacist site that has been booted by both Go. Daddy and Google and has had a hard time finding a home. The most notable conspiracy theory was floated by neo- Nazi hacker Weev, who said that he had hacked Kessler’s account. Weev, whose real name is Andrew Auernheimer, published a post on the social networking site Gab about two hours after Kessler’s tweet, taking credit for the hateful message. But Weev is known for making outlandish claims and so far there’s been no evidence presented to substantiate the claim that he hacked Kessler. Other white supremacists who attended the rally in Charlottesville, like Richard Spencer, said that they would no longer associate with Kessler. Spencer, who was a mentor to Trump White House advisor Stephen Miller at Duke University, came to prominence after leading a neo- Nazi rally in November of 2. ![]() ![]() Hail Trump, hail our people, hail victory!”“Hail victory” is English for the German Nazi slogan “sieg heil.”White supremacist Baked Alaska, who also attended the rally and posted video of himself shouting “hail victory,” also sought to distance himself from Kessler, disavowing “actual racial hatred.” Baked Alaska’s real name is Tim Gionet. It’s not clear what Gionet means by the tweet, as marching in a neo- Nazi rally seems to be the very definition of “spewing actual racial hatred.” Gionet has seemingly been much more open to the cause of non- violence since getting maced at the rally in Charlottesville, but not open enough to denounce the cause of white supremacy. He also doesn’t seem to understand that things like Holocaust denial and retweeting “Hitler did nothing wrong,” is itself a call to violence. But there was a broad mix of responses to Kessler’s tweet on Gab, the social network of choice for white supremacists who’ve been banned from Twitter. Some on the network insisted that Kessler’s tweet was proof that he’s actually a “paid plant” controlled by the US government to make white supremacists look bad. Some so- called alt- right activists have said that Kessler is a secret Barack Obama supporter and even wanted Hillary Clinton to win, basically the worst things you can say in the white supremacist community about other neo- Nazis. Download PoKeMoN games all Gen Pokemon® X and Y, Red Blue and & Sun and Moon 【•】 Mac and PC compatible iPhone/Android emulator roms direct Torrent link ⌚. A senior US official has admitted to being the source behind a claim that the FCC was “hacked” in 2014 during the net neutrality debate. Internally, however, the. Others said that Heather Heyer herself was some kind of government agent. Several white supremacists insisted that anyone distancing themselves from Kessler were cucks and traitors to the cause of white nationalism. The term cucks has been popularized in the Trump- supporting community as a derivative of “cuckold” and is meant to be a derogatory term for other conservatives. One alt- right You. At its press event yesterday, Apple announced a slew of new products, including a trio of new iPhones (like the $999 iPhone X), an LTE-equipped Apple Watch, and an.Tube and podcasting personality, Tara Mc. Carthy, said on Gab that she understood why some people would “lay low” for the time being, but hinted that the idea of disavowing the cause made one a traitor. Gizmodo has reached out to Jason Kessler for comment and will update this post if we hear back. Heather Heyer couldn’t be reached for comment because she was killed by a neo- Nazi terrorist. Update, 7: 4. 8am ET: Jason Kessler has now deleted the tweet, but has not responded to Gizmodo’s request for comment. Update, 7: 5. 5am ET: Kessler just tweeted, apologizing for the tweet about Heather Heyer and claiming that he was hacked last night. We have yet to see any evidence to confirm or deny whether Kessler was hacked, but he has previous used the term “payback time” in tweets from June 1. And platforming militantly anti- white academics from #UVA. Now its payback time. The #altright & right wing will have its day to speak.”He also tweeted about the Unite the Right rally using the tweet above. Update, 8: 0. 4am ET: And now Kessler just deleted his tweet saying he was hacked for some reason. But I got a screenshot. I still haven’t heard from Kessler directly. Update, 1. 0: 4. 9am ET: Kessler just sent out two tweets where he no longer claims that he was hacked and instead blames his tweets about Heather Heyer on stress and a combination of alcohol, sleep medication, and anti- anxiety drugs. Update, 1. 2: 5. 4pm ET: It appears that Jason Kessler has either deactivated or permanently deleted his Twitter account. I’ve uploaded screenshots above for all the tweets that were previously embedded. These Are the Devices That Support i. OS 1. 1 and mac. OS High Sierra. At its press event yesterday, Apple announced a slew of new products, including a trio of new i. Phones (like the $9. Phone X), an LTE- equipped Apple Watch, and an Apple TV capable of displaying movies in 4. K HDR. The announcements also coincided with some software update news, namely release dates for i. OS 1. 1 and mac. OS High Sierra, updated versions of Apple’s mobile and desktop operating systems, respectively. The i. Phone X’s full- screen display may be the first thing you notice about the new device, but…Read more Devices Supporting i. OS 1. 1i. OS 1. 1 will be available for launch on September 1. Phone and i. Pad with the new software. Unfortunately, not every phone is supported. Phones from the i. Phone 7 to the i. Phone 5s are capable of running i. OS 1. 1. That leaves older devices, including the original i. Phone 5 and i. Phone 5c (released in 2. Here’s the official list of supported i. Phones: i. Phone 7i. Phone 7 Plusi. Phone 6si. Phone 6s Plusi. Phone 6i. Phone 6 Plusi. Phone SEi. Phone 5s. As for the i. Pad, i. OS 1. 1 supports devices in both the i. Pad and i. Pad mini line of tablets. The oldest mini supported is the i. Pad mini 2, while the oldest i. Pad supported is the i. Pad Air, both of which debuted in 2. That means i. OS 1. Pad, the first i. Pad with a high- resolution Retina display. Here’s the official list of supported i. Pads: 1. 2. 9- inch i. Pad Pro (2nd generation)1. Pad Pro (1st generation)i. Pad Pro (1. 0. 5- inch)i. Pad Pro (9. 7- inch)i. Pad Air 2i. Pad Airi. Pad (5th generation)i. Pad mini 4i. Pad mini 3i. Pad mini 2. The only other device supporting i. OS 1. 1 is the sixth- generation i. Pod touch, released in 2. It’s also the only currently sold i. OS device that doesn’t have Touch. ID support so, you know, maybe don’t get that one. Devices Supporting mac. OS High Sierra. Apple’s mac. OS High Sierra for its desktop computers is also getting ready to debut September 2. While its predecessor, Sierra, was packed with new features like Siri integration, Night Shift software for easing eye strain during late- night use, and Apple’s solid- state- optimized Apple File System, High Sierra’s changes are mostly under the hood. It supports the same devices supported by Sierra, so if you’ve got the old version you’re good to go when upgrading to its successor. According to Apple, “All Macs introduced in mid 2. Mac. Book and i. Mac computers introduced in late 2. Here are the devices supported by mac. OS High Sierra: i. Mac: (late 2. 00. Mac. Book/Mac. Book (Retina): (late 2. Mac. Book Pro: (mid- 2. Mac. Book Air: (late 2. Mac Mini: (mid- 2. Mac Pro: (mid- 2. Mac Pro (2. 01. 7)If you’re not sure the age of your Mac, there’s a really simple way to check. Just click the Apple logo in the top- left corner of your display, select “About This Mac,” and take a look at the name of your device, which should include its manufacturing year. You can also use Apple’s serial number identification page that provides you with even more information about your Mac, such as its purchase date, Apple. Care Protection Plan status, and repair coverage. If you find your Mac or i. OS device unable to receive the new software, don’t fret too much. Newer software takes advantage of newer hardware, meaning your older device might simply find the update too intensive for its outdated hardware. It’s a bummer, but at least you can turn your vintage tech into cash by trading it in. |
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October 2017
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